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Science 29 August 1986:
Vol. 233. no. 4767, pp. 972 - 974
DOI: 10.1126/science.2426781
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Articles

Science, Vol 233, Issue 4767, 972-974
Copyright © 1986 by American Association for the Advancement of Science

articles

Measurement of single channel currents from cardiac gap junctions

RD Veenstra and RL DeHaan

Cardiac gap junctions consist of arrays of integral membrane proteins joined across the intercellular cleft at points of cell-to-cell contact. These junctional proteins are thought to form pores through which ions can diffuse from cytosol to cytosol. By monitoring whole-cell currents in pairs of embryonic heart cells with two independent patch-clamp circuits, the properties of single gap junction channels have been investigated. These channels had a conductance of about 165 picosiemens and underwent spontaneous openings and closings that were independent of voltage. Channel activity and macroscopic junctional conductance were both decreased by the uncoupling agent 1-octanol.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Gap junction heterogeneity in reentrant ventricular tachycardia.
R. D. Veenstra (2006)
Cardiovasc Res 72, 196-197
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Cardiac Ionic Currents and Acute Ischemia: From Channels to Arrhythmias.
E. Carmeliet (1999)
Physiol Rev 79, 917-1017
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The Sleep-inducing Lipid Oleamide Deconvolutes Gap Junction Communication and Calcium Wave Transmission in Glial Cells.
X. Guan, B. F. Cravatt, G. R. Ehring, J. E. Hall, D. L. Boger, R. A. Lerner, and N. B. Gilula (1997)
J. Cell Biol. 139, 1785-1792
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Unique Conductance, Gating, and Selective Permeability Properties of Gap Junction Channels Formed by Connexin40.
D. A. Beblo, H.-Z. Wang, E. C. Beyer, E. M. Westphale, and R. D. Veenstra (1995)
Circ. Res. 77, 813-822
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Mutations of the Connexin43 Gap-Junction Gene in Patients with Heart Malformations and Defects of Laterality.
S. H. Britz-Cunningham, M. M. Shah, C. W. Zuppan, and W. H. Fletcher (1995)
N. Engl. J. Med. 332, 1323-1330
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Clusters of coupled neuroblasts in embryonic neocortex.
J. Lo Turco and A. Kriegstein (1991)
Science 252, 563-566
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Identification of synaptophysin as a hexameric channel protein of the synaptic vesicle membrane.
L Thomas, K Hartung, D Langosch, H Rehm, E Bamberg, W. Franke, and H Betz (1988)
Science 242, 1050-1053
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Ryanodine receptor of skeletal muscle is a gap junction-type channel.
J Ma, M Fill, C. Knudson, K. Campbell, and R Coronado (1988)
Science 242, 99-102
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Gap junctional conductance and permeability are linearly related.
V Verselis, R. White, D. Spray, and M. Bennett (1986)
Science 234, 461-464
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