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Science 8 November 1991:
Vol. 254. no. 5033, pp. 866 - 868
DOI: 10.1126/science.1658937
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Articles

Science, Vol 254, Issue 5033, 866-868
Copyright © 1991 by American Association for the Advancement of Science

articles

A phosphorylation site in the Na+ channel required for modulation by protein kinase C

JW West, R Numann, BJ Murphy, T Scheuer, and WA Catterall

Department of Pharmacology, University of Washington, Seattle 98195.

Voltage-gated sodium channels are responsible for generation of action potentials in excitable cells. Activation of protein kinase C slows inactivation of sodium channels and reduces peak sodium currents. Phosphorylation of a single residue, serine 1506, that is located in the conserved intracellular loop between domains III and IV and is involved in inactivation of the sodium channel, is required for both modulatory effects. Mutant sodium channels lacking this phosphorylation site have normal functional properties in unstimulated cells but do not respond to activation of protein kinase C. Phosphorylation of this conserved site in sodium channel alpha subunits may regulate electrical activity in a wide range of excitable cells.


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