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Science 12 July 1996:
Vol. 273. no. 5272, pp. 248 - 251
DOI: 10.1126/science.273.5272.248
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Reports

Bipartite Ca2+-Binding Motif in C2 Domains of Synaptotagmin and Protein Kinase C

Xuguang Shao, Bazbek A. Davletov, * R. Bryan Sutton, Thomas C. Südhof, Josep Rizo dagger

C2 domains are found in many proteins involved in membrane traffic or signal transduction. Although C2 domains are thought to bind calcium ions, the structural basis for calcium binding is unclear. Analysis of calcium binding to C2 domains of synaptotagmin I and protein kinase C-beta by nuclear magnetic resonance spectroscopy revealed a bipartite calcium-binding motif that involves the coordination of two calcium ions by five aspartate residues located on two separate loops. Sequence comparisons indicated that this may be a widely used calcium-binding motif, designated here as the C2 motif.

X. Shao and J. Rizo, Department of Pharmacology, and R. B. Sutton, Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75235, USA.
B. A. Davletov and T. C. Südhof, Department of Molecular Genetics and Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75235, USA.
* Present address: Department of Biochemistry, Imperial College of Science, Technology and Medicine, London SW7 2AY, UK.
dagger To whom correspondence should be addressed.


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