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Science 12 July 1996:
Vol. 273. no. 5272, pp. 252 - 255
DOI: 10.1126/science.273.5272.252
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Reports

Platelet-Mediated Lymphocyte Delivery to High Endothelial Venules

Thomas G. Diacovo, * Kamal D. Puri, * R. Aaron Warnock, Timothy A. Springer, Ulrich H. von Andrian dagger

Circulating lymphocytes gain access to lymph nodes owing to their ability to initiate rolling along specialized high endothelial venules (HEVs). One mechanism of rolling involves L-selectin binding to peripheral node addressin (PNAd) on HEVs. Activated platelets are shown to bind to circulating lymphocytes and to mediate rolling in HEVs, in vivo, through another molecule, P-selectin, which also interacts with PNAd. In vitro, activated platelets enhanced tethering of lymphocytes to PNAd and sustained lymphocyte rolling, even in the absence of functional L-selectin. Thus, a platelet pathway operating through P-selectin provides a second mechanism for lymphocyte delivery to HEVs.

T. G. Diacovo, Center for Blood Research and Department of Cardiology, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA, and Division of Newborn Medicine, Department of Pediatrics, Tufts University School of Medicine, Boston, MA 02111, USA.
K. D. Puri, T. A. Springer, U. H. von Andrian, Center for Blood Research and Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA.
R. A. Warnock, Department of Pathology, Stanford University, Stanford, CA 94305, USA.
* These authors contributed equally to this work.
dagger To whom correspondence should be addressed at the Center for Blood Research, 200 Longwood Avenue, Boston, MA 02115, USA. E-mail: uva@cbr.med.harvard.edu


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