Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 29 August 1986:
Vol. 233. no. 4767, pp. 967 - 972
DOI: 10.1126/science.3016898
Prev | Table of Contents | Next

Articles

Science, Vol 233, Issue 4767, 967-972
Copyright © 1986 by American Association for the Advancement of Science

articles

Insulin-stimulated hydrolysis of a novel glycolipid generates modulators of cAMP phosphodiesterase

AR Saltiel, JA Fox, P Sherline, and P Cuatrecasas

Insulin action may involve the intracellular generation of low molecular weight substances that modulate certain key enzymes. The production of two substances that regulate the activity of adenosine 3',5'-monophosphate phosphodiesterase was evaluated in cultured myocytes by incorporation of radiolabeled precursors. Insulin caused the rapid hydrolysis of a chemically undefined membrane glycolipid, resulting in the production of two related complex carbohydrates as well as diacylglycerol. Both the glycolipid precursor and the aqueous products were monitored by labeling with radioactive inositol and glucosamine. Depletion of the labeled precursor and the appearance of labeled water-soluble products and diacylglycerol occurred within 30 seconds after hormone treatment and was followed by rapid resynthesis of the precursor. The aqueous products that were radioactively labeled appeared chromatographically and electrophoretically identical to phosphodiesterase modulating activities produced by insulin from the same cells. The purified radiolabeled and bioactive substances had similar chemical properties. Hydrolysis of the glycolipid precursor and subsequent generation of products could be reproduced by incubation of extracted lipids with a phosphatidylinositol-specific phospholipase C. These studies suggest that insulin stimulates an endogenous, selective phospholipase C activity that hydrolyzes a novel glycolipid, resulting in the generation of a complex carbohydrate-phosphate substance containing inositol and glucosamine that may mediate some of the actions of the hormone.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Insulin-like Growth Factors Mediate Heterotrimeric G Protein-dependent ERK1/2 Activation by Transactivating Sphingosine 1-Phosphate Receptors.
H. M. El-Shewy, K. R. Johnson, M.-H. Lee, A. A. Jaffa, L. M. Obeid, and L. M. Luttrell (2006)
J. Biol. Chem. 281, 31399-31407
   Abstract »    Full Text »    PDF »
Expression of De-N-acetyl-gangliosides in Human Melanoma Cells Is Induced by Genistein or Nocodazole.
E. R. Sjoberg, R. Chammas, H. Ozawa, I. Kawashima, K.-H. Khoo, H. R. Morris, A. Dell, T. Tai, and A. Varki (1995)
J. Biol. Chem. 270, 2921-2930
   Abstract »    Full Text »    PDF »
Glycosylphosphatidylinositol: a candidate system for interleukin-2 signal transduction.
D. Eardley and M. Koshland (1991)
Science 251, 78-81
   Abstract »    PDF »
Review: The Phospholipid/Arachidonic Acid Second Messenger System: Its Possible Role in Physiology and Pathophysiology of Metabolism.
W. H. Hartl and R. R. Wolfe (1990)
JPEN J Parenter Enteral Nutr 14, 416-427
   Abstract »    PDF »
Short-term Electrophysiological Actions of Insulin on Aplysia Neurons: Identification of a Possible Novel Modulatory Second-messenger Mechanism.
J.H. Schwartz, E. Shapiro, S.D. Brown, and A.R. Saltiel (1990)
Cold Spring Harb Symp Quant Biol 55, 95-100
   Abstract »    PDF »
Insulin-stimulated release of lipoprotein lipase by metabolism of its phosphatidylinositol anchor.
B. Chan, M. Lisanti, E Rodriguez-Boulan, and A. Saltiel (1988)
Science 241, 1670-1672
   Abstract »    PDF »
Phosphatidylinositol-glycan anchors of membrane proteins: potential precursors of insulin mediators.
G Romero, L Luttrell, A Rogol, K Zeller, E Hewlett, and J Larner (1988)
Science 240, 509-511
   Abstract »    PDF »
Glycosyl-phosphatidylinositol moiety that anchors Trypanosoma brucei variant surface glycoprotein to the membrane.
M. Ferguson, S. Homans, R. Dwek, and T. Rademacher (1988)
Science 239, 753-759
   Abstract »    PDF »
Structural and functional roles of glycosyl-phosphatidylinositol in membranes.
M. Low and A. Saltiel (1988)
Science 239, 268-275
   Abstract »    PDF »
Role of Glycosyl Phosphoinositides in Insulin Action.
A.R. Saltiel, D.G. Osterman, and J.C. Darnell (1988)
Cold Spring Harb Symp Quant Biol 53, 955-963
   Abstract »    PDF »
After insulin binds.
O. Rosen (1987)
Science 237, 1452-1458
   Abstract »    PDF »
Ras p21 as a potential mediator of insulin action in Xenopus oocytes.
L. Korn, C. Siebel, F McCormick, and R. Roth (1987)
Science 236, 840-843
   Abstract »    PDF »
Insulin rapidly increases diacylglycerol by activating de novo phosphatidic acid synthesis.
R. Farese, T. Konda, J. Davis, M. Standaert, R. Pollet, and D. Cooper (1987)
Science 236, 586-589
   Abstract »    PDF »
Mechanisms of Receptor-mediated Transmembrane Communication.
L. Ellis, D.O. Morgan, E. Clauser, M. Edery, S.-M. Jong, L.-H. Wang, R.A. Roth, and W.J. Rutter (1986)
Cold Spring Harb Symp Quant Biol 51, 773-784
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)