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Science 29 August 1986:
Vol. 233. no. 4767, pp. 972 - 974
DOI: 10.1126/science.2426781
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Articles

Science, Vol 233, Issue 4767, 972-974
Copyright © 1986 by American Association for the Advancement of Science

articles

Measurement of single channel currents from cardiac gap junctions

RD Veenstra and RL DeHaan

Cardiac gap junctions consist of arrays of integral membrane proteins joined across the intercellular cleft at points of cell-to-cell contact. These junctional proteins are thought to form pores through which ions can diffuse from cytosol to cytosol. By monitoring whole-cell currents in pairs of embryonic heart cells with two independent patch-clamp circuits, the properties of single gap junction channels have been investigated. These channels had a conductance of about 165 picosiemens and underwent spontaneous openings and closings that were independent of voltage. Channel activity and macroscopic junctional conductance were both decreased by the uncoupling agent 1-octanol.


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L Thomas, K Hartung, D Langosch, H Rehm, E Bamberg, W. Franke, and H Betz (1988)
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Ryanodine receptor of skeletal muscle is a gap junction-type channel.
J Ma, M Fill, C. Knudson, K. Campbell, and R Coronado (1988)
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Gap junctional conductance and permeability are linearly related.
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