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Science 15 August 1997:
Vol. 277. no. 5328, pp. 949 - 952
DOI: 10.1126/science.277.5328.949
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Reports

Conversion by Peyer's Patch Lymphocytes of Human Enterocytes into M Cells that Transport Bacteria

Sophie Kernéis, * Anna Bogdanova, dagger Jean-Pierre Kraehenbuhl, Eric Pringault *ddagger

The epithelium that lines the gut is impermeable to macromolecules and microorganisms, except in Peyer's patches (PPs), where the lymphoid follicle-associated epithelium (FAE) contains M cells that transport antigens and microorganisms. A cultured system that reproduces the main characteristics of FAE and M cells was established by cultivation of PP lymphocytes with the differentiated human intestinal cell line Caco-2. Lymphocytes settled into the epithelial monolayer, inducing reorganization of the brush border and a temperature-dependent transport of particles and Vibrio cholerae. This model system could prove useful for intestinal physiology, vaccine research, and drug delivery studies.

Swiss Institute for Experimental Cancer Research, and Institute of Biochemistry, University of Lausanne, CH-1066 Epalinges-Lausanne, Switzerland.
*   Present address: Department of Bacteriology and Mycology, Pasteur Institute, 28 rue du Dr Roux, 75724 Paris Cedex 15, France.

dagger    Present address: INSERM U445, 22 rue Méchain, 75014 Paris, France.

ddagger    To whom correspondence should be addressed. E-mail: epringau{at}pasteur.fr

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