Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Originally published in Science Express on 6 June 2002
Science 26 July 2002:
Vol. 297. no. 5581, pp. 612 - 615
DOI: 10.1126/science.1072309
Prev | Table of Contents | Next

Reports

Role of Formins in Actin Assembly: Nucleation and Barbed-End Association

David Pruyne,1* Marie Evangelista,2* Changsong Yang,3 Erfei Bi,4 Sally Zigmond,3 Anthony Bretscher,1dagger Charles Boone25dagger

Nucleation of branched actin filaments by the Arp2/3 complex is a conserved process in eukaryotic cells, yet the source of unbranched actin filaments has remained obscure. In yeast, formins stimulate assembly of actin cables independently of Arp2/3. Here, the conserved core of formin homology domains 1 and 2 of Bni1p (Bni1pFH1FH2) was found to nucleate unbranched actin filaments in vitro. Bni1pFH2 provided the minimal region sufficient for nucleation. Unique among actin nucleators, Bni1pFH1FH2 remained associated with the growing barbed ends of filaments. This combination of properties suggests a direct role for formins in regulating nucleation and polarization of unbranched filamentous actin structures.

1 Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA.
2 Department of Biology, Queen's University, Kingston, Ontario, K7L 3N6 Canada.
3 Biology Department, University of Pennsylvania, Philadelphia, PA 19104-6018, USA.
4 Department of Cell and Developmental Biology, University of Pennsylvania, Philadelphia, PA 19104, USA.
5 Banting and Best Department of Medical Research and Department of Molecular and Medical Genetics, University of Toronto, Toronto, Ontario, M5G 1L6 Canada.
*   These authors contributed equally to this work.

dagger    To whom correspondence should be addressed. E-mail: apb5{at}cornell.edu (A.B.), charlie.boone{at}utoronto.ca (C.B.)

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Rapid formin-mediated actin-filament elongation is essential for polarized plant cell growth.
L. Vidali, P. A. C. van Gisbergen, C. Guerin, P. Franco, M. Li, G. M. Burkart, R. C. Augustine, L. Blanchoin, and M. Bezanilla (2009)
PNAS 106, 13341-13346
   Abstract »    Full Text »    PDF »
Formin1 disruption confers oligodactylism and alters Bmp signaling.
F. Zhou, P. Leder, A. Zuniga, and M. Dettenhofer (2009)
Hum. Mol. Genet. 18, 2472-2482
   Abstract »    Full Text »    PDF »
The Yeast AAA+ Chaperone Hsp104 Is Part of a Network That Links the Actin Cytoskeleton with the Inheritance of Damaged Proteins.
P. Tessarz, M. Schwarz, A. Mogk, and B. Bukau (2009)
Mol. Cell. Biol. 29, 3738-3745
   Abstract »    Full Text »    PDF »
Reactive Oxygen Species Regulate a Slingshot-Cofilin Activation Pathway.
J.-S. Kim, T. Y. Huang, and G. M. Bokoch (2009)
Mol. Biol. Cell 20, 2650-2660
   Abstract »    Full Text »    PDF »
Polarized Growth in Budding Yeast in the Absence of a Localized Formin.
L. Gao and A. Bretscher (2009)
Mol. Biol. Cell 20, 2540-2548
   Abstract »    Full Text »    PDF »
Energetic Requirements for Processive Elongation of Actin Filaments by FH1FH2-formins.
A. S. Paul and T. D. Pollard (2009)
J. Biol. Chem. 284, 12533-12540
   Abstract »    Full Text »    PDF »
Genetic and cell biological analysis of integrin outside-in signaling.
K. R. Legate, S. A. Wickstrom, and R. Fassler (2009)
Genes & Dev. 23, 397-418
   Abstract »    Full Text »    PDF »
Incompatibility with Formin Cdc12p Prevents Human Profilin from Substituting for Fission Yeast Profilin: INSIGHTS FROM CRYSTAL STRUCTURES OF FISSION YEAST PROFILIN.
O. C. Ezezika, N. S. Younger, J. Lu, D. A. Kaiser, Z. A. Corbin, B. J. Nolen, D. R. Kovar, and T. D. Pollard (2009)
J. Biol. Chem. 284, 2088-2097
   Abstract »    Full Text »    PDF »
Specificity of Interactions between mDia Isoforms and Rho Proteins.
M. Lammers, S. Meyer, D. Kuhlmann, and A. Wittinghofer (2008)
J. Biol. Chem. 283, 35236-35246
   Abstract »    Full Text »    PDF »
Origins and Evolution of the Formin Multigene Family That Is Involved in the Formation of Actin Filaments.
D. Chalkia, N. Nikolaidis, W. Makalowski, J. Klein, and M. Nei (2008)
Mol. Biol. Evol. 25, 2717-2733
   Abstract »    Full Text »    PDF »
Dissecting the involvement of formins in Bud6p-mediated cortical capture of microtubules in S. cerevisiae.
N. Delgehyr, C. S. J. Lopes, C. A. Moir, S. M. Huisman, and M. Segal (2008)
J. Cell Sci. 121, 3803-3814
   Abstract »    Full Text »    PDF »
Formin-Dependent Synaptic Growth: Evidence That Dlar Signals via Diaphanous to Modulate Synaptic Actin and Dynamic Pioneer Microtubules.
C. Pawson, B. A. Eaton, and G. W. Davis (2008)
J. Neurosci. 28, 11111-11123
   Abstract »    Full Text »    PDF »
Dynamic localization of yeast Fus2p to an expanding ring at the cell fusion junction during mating.
J. M. Paterson, C. A. Ydenberg, and M. D. Rose (2008)
J. Cell Biol. 181, 697-709
   Abstract »    Full Text »    PDF »
G-actin regulates rapid induction of actin nucleation by mDia1 to restore cellular actin polymers.
C. Higashida, S. Suetsugu, T. Tsuji, J. Monypenny, S. Narumiya, and N. Watanabe (2008)
J. Cell Sci. 121, 3403-3412
   Abstract »    Full Text »    PDF »
The formin mDia2 stabilizes microtubules independently of its actin nucleation activity.
F. Bartolini, J. B. Moseley, J. Schmoranzer, L. Cassimeris, B. L. Goode, and G. G. Gundersen (2008)
J. Cell Biol. 181, 523-536
   Abstract »    Full Text »    PDF »
Entamoeba histolytica Encodes Unique Formins, a Subset of Which Regulates DNA Content and Cell Division.
S. Majumder and A. Lohia (2008)
Infect. Immun. 76, 2368-2378
   Abstract »    Full Text »    PDF »
Regulation of Actin Assembly Associated With Protrusion and Adhesion in Cell Migration.
C. Le Clainche and M.-F. Carlier (2008)
Physiol Rev 88, 489-513
   Abstract »    Full Text »    PDF »
Analysis of Unregulated Formin Activity Reveals How Yeast Can Balance F-Actin Assembly between Different Microfilament-based Organizations.
L. Gao and A. Bretscher (2008)
Mol. Biol. Cell 19, 1474-1484
   Abstract »    Full Text »    PDF »
G1/S Cyclin-dependent Kinase Regulates Small GTPase Rho1p through Phosphorylation of RhoGEF Tus1p in Saccharomyces cerevisiae.
K. Kono, S. Nogami, M. Abe, M. Nishizawa, S. Morishita, D. Pellman, and Y. Ohya (2008)
Mol. Biol. Cell 19, 1763-1771
   Abstract »    Full Text »    PDF »
Arp2/3 Complex Is Important for Filopodia Formation, Growth Cone Motility, and Neuritogenesis in Neuronal Cells.
F. Korobova and T. Svitkina (2008)
Mol. Biol. Cell 19, 1561-1574
   Abstract »    Full Text »    PDF »
Biochemical Characterization of the Rho GTPase-regulated Actin Assembly by Diaphanous-related Formins, mDia1 and Daam1, in Platelets.
T. Higashi, T. Ikeda, R. Shirakawa, H. Kondo, M. Kawato, M. Horiguchi, T. Okuda, K. Okawa, S. Fukai, O. Nureki, et al. (2008)
J. Biol. Chem. 283, 8746-8755
   Abstract »    Full Text »    PDF »
WASP family members and formin proteins coordinate regulation of cell protrusions in carcinoma cells.
C. Sarmiento, W. Wang, A. Dovas, H. Yamaguchi, M. Sidani, M. El-Sibai, V. DesMarais, H. A. Holman, S. Kitchen, J. M. Backer, et al. (2008)
J. Cell Biol. 180, 1245-1260
   Abstract »    Full Text »    PDF »
Crystal structure of human DAAM1 formin homology 2 domain..
M. Yamashita, T. Higashi, S. Suetsugu, Y. Sato, T. Ikeda, R. Shirakawa, T. Kita, T. Takenawa, H. Horiuchi, S. Fukai, et al. (2007)
Genes Cells 12, 1255-1265
   Abstract »    Full Text »    PDF »
The Diaphanous Inhibitory Domain/Diaphanous Autoregulatory Domain Interaction Is Able to Mediate Heterodimerization between mDia1 and mDia2.
S. J. Copeland, B. J. Green, S. Burchat, G. A. Papalia, D. Banner, and J. W. Copeland (2007)
J. Biol. Chem. 282, 30120-30130
   Abstract »    Full Text »    PDF »
mDia2 regulates actin and focal adhesion dynamics and organization in the lamella for efficient epithelial cell migration.
S. L. Gupton, K. Eisenmann, A. S. Alberts, and C. M. Waterman-Storer (2007)
J. Cell Sci. 120, 3475-3487
   Abstract »    Full Text »    PDF »
Regulation of the Formin for3p by cdc42p and bud6p.
S. G. Martin, S. A. Rincon, R. Basu, P. Perez, and F. Chang (2007)
Mol. Biol. Cell 18, 4155-4167
   Abstract »    Full Text »    PDF »
Stable Preanaphase Spindle Positioning Requires Bud6p and an Apparent Interaction between the Spindle Pole Bodies and the Neck.
B. K. Haarer, A. H. Helfant, S. A. Nelson, J. A. Cooper, and D. C. Amberg (2007)
Eukaryot. Cell 6, 797-807
   Abstract »    Full Text »    PDF »
Yeast Formins Bni1 and Bnr1 Utilize Different Modes of Cortical Interaction during the Assembly of Actin Cables.
S. M. Buttery, S. Yoshida, and D. Pellman (2007)
Mol. Biol. Cell 18, 1826-1838
   Abstract »    Full Text »    PDF »
Requirement for the Polarisome and Formin Function in Ssk2p-Mediated Actin Recovery From Osmotic Stress in Saccharomyces cerevisiae.
B. T. Bettinger, M. G. Clark, and D. C. Amberg (2007)
Genetics 175, 1637-1648
   Abstract »    Full Text »    PDF »
Avl9p, a Member of a Novel Protein Superfamily, Functions in the Late Secretory Pathway.
E. Harsay and R. Schekman (2007)
Mol. Biol. Cell 18, 1203-1219
   Abstract »    Full Text »    PDF »
How ATP Hydrolysis Controls Filament Assembly from Profilin-Actin: IMPLICATION FOR FORMIN PROCESSIVITY.
S. Romero, D. Didry, E. Larquet, N. Boisset, D. Pantaloni, and M.-F. Carlier (2007)
J. Biol. Chem. 282, 8435-8445
   Abstract »    Full Text »    PDF »
Central Roles of Small GTPases in the Development of Cell Polarity in Yeast and Beyond.
H.-O. Park and E. Bi (2007)
Microbiol. Mol. Biol. Rev. 71, 48-96
   Abstract »    Full Text »    PDF »
Hyphal Growth: a Tale of Motors, Lipids, and the Spitzenkorper.
G. Steinberg (2007)
Eukaryot. Cell 6, 351-360
   Full Text »    PDF »
A Zn-finger/FH2-domain containing protein, FOZI-1, acts redundantly with CeMyoD to specify striated body wall muscle fates in the Caenorhabditis elegans postembryonic mesoderm.
N. M. Amin, K. Hu, D. Pruyne, D. Terzic, A. Bretscher, and J. Liu (2007)
Development 134, 19-29
   Abstract »    Full Text »    PDF »
Roles of type II myosin and a tropomyosin isoform in retrograde actin flow in budding yeast.
T. M. Huckaba, T. Lipkin, and L. A. Pon (2006)
J. Cell Biol. 175, 957-969
   Abstract »    Full Text »    PDF »
Chlamydial TARP is a bacterial nucleator of actin.
T. J. Jewett, E. R. Fischer, D. J. Mead, and T. Hackstadt (2006)
PNAS 103, 15599-15604
   Abstract »    Full Text »    PDF »
Yeast-to-Hyphal Transition Triggers Formin-dependent Golgi Localization to the Growing Tip in Candida albicans.
P. C.G. Rida, A. Nishikawa, G. Y. Won, and N. Dean (2006)
Mol. Biol. Cell 17, 4364-4378
   Abstract »    Full Text »    PDF »
The Yeast Actin Cytoskeleton: from Cellular Function to Biochemical Mechanism.
J. B. Moseley and B. L. Goode (2006)
Microbiol. Mol. Biol. Rev. 70, 605-645
   Abstract »    Full Text »    PDF »
An unusual Zn-finger/FH2 domain protein controls a left/right asymmetric neuronal fate decision in C. elegans.
R. J. Johnston Jr, J. W. Copeland, M. Fasnacht, J. F. Etchberger, J. Liu, B. Honig, and O. Hobert (2006)
Development 133, 3317-3328
   Abstract »    Full Text »    PDF »
Effects of Human Deafness {gamma}-Actin Mutations (DFNA20/26) on Actin Function.
K. E. Bryan, K.-K. Wen, M. Zhu, N. D. Rendtorff, M. Feldkamp, L. Tranebjaerg, K. H. Friderici, and P. A. Rubenstein (2006)
J. Biol. Chem. 281, 20129-20139
   Abstract »    Full Text »    PDF »
Identification of Novel Mutations in ACT1 and SLA2 That Suppress the Actin-Cable-Overproducing Phenotype Caused by Overexpression of a Dominant Active Form of Bni1p in Saccharomyces cerevisiae.
S. Yoshiuchi, T. Yamamoto, H. Sakane, J. Kadota, J. Mochida, M. Asaka, and K. Tanaka (2006)
Genetics 173, 527-539
   Abstract »    Full Text »    PDF »
Formins Regulate Actin Filament Flexibility through Long Range Allosteric Interactions.
B. Bugyi, G. Papp, G. Hild, D. Lorinczy, E. M. Nevalainen, P. Lappalainen, B. Somogyi, and M. Nyitrai (2006)
J. Biol. Chem. 281, 10727-10736
   Abstract »    Full Text »    PDF »
Spatial dynamics of receptor-mediated endocytic trafficking in budding yeast revealed by using fluorescent {alpha}-factor derivatives.
J. Y. Toshima, J. Toshima, M. Kaksonen, A. C. Martin, D. S. King, and D. G. Drubin (2006)
PNAS 103, 5793-5798
   Abstract »    Full Text »    PDF »
Regulated Phosphorylation of Budding Yeast's Essential Myosin V Heavy Chain, Myo2p.
A. Legesse-Miller, S. Zhang, F. H. Santiago-Tirado, C. K. Van Pelt, and A. Bretscher (2006)
Mol. Biol. Cell 17, 1812-1821
   Abstract »    Full Text »    PDF »
Expression Profiling of a Hypercontraction-induced Myopathy in Drosophila Suggests a Compensatory Cytoskeletal Remodeling Response.
E. S. Montana and J. T. Littleton (2006)
J. Biol. Chem. 281, 8100-8109
   Abstract »    Full Text »    PDF »
The Drosophila formin DAAM regulates the tracheal cuticle pattern through organizing the actin cytoskeleton.
T. Matusek, A. Djiane, F. Jankovics, D. Brunner, M. Mlodzik, and J. Mihaly (2006)
Development 133, 957-966
   Abstract »    Full Text »    PDF »
Mutational Analysis of Stubble-stubbloid Gene Structure and Function in Drosophila Leg and Bristle Morphogenesis.
A. S. Hammonds and J. W. Fristrom (2006)
Genetics 172, 1577-1593
   Abstract »    Full Text »    PDF »
Biochemical Characterization of the Diaphanous Autoregulatory Interaction in the Formin Homology Protein FHOD1.
A. Schonichen, M. Alexander, J. E. Gasteier, F. E. Cuesta, O. T. Fackler, and M. Geyer (2006)
J. Biol. Chem. 281, 5084-5093
   Abstract »    Full Text »    PDF »
The Basic Region of the Diaphanous-autoregulatory Domain (DAD) Is Required for Autoregulatory Interactions with the Diaphanous-related Formin Inhibitory Domain.
B. J. Wallar, B. N. Stropich, J. A. Schoenherr, H. A. Holman, S. M. Kitchen, and A. S. Alberts (2006)
J. Biol. Chem. 281, 4300-4307
   Abstract »    Full Text »    PDF »
Regulation of mitochondria distribution by RhoA and formins.
A. A. Minin, A. V. Kulik, F. K. Gyoeva, Y. Li, G. Goshima, and V. I. Gelfand (2006)
J. Cell Sci. 119, 659-670
   Abstract »    Full Text »    PDF »
From Function to Shape: A Novel Role of a Formin in Morphogenesis of the Fungus Ashbya gossypii.
H.-P. Schmitz, A. Kaufmann, M. Kohli, P. P. Laissue, and P. Philippsen (2006)
Mol. Biol. Cell 17, 130-145
   Abstract »    Full Text »    PDF »
Sequence and Comparative Genomic Analysis of Actin-related Proteins.
J. Muller, Y. Oma, L. Vallar, E. Friederich, O. Poch, and B. Winsor (2005)
Mol. Biol. Cell 16, 5736-5748
   Abstract »    Full Text »    PDF »
Tum/RacGAP50C provides a critical link between anaphase microtubules and the assembly of the contractile ring in Drosophila melanogaster.
M. Zavortink, N. Contreras, T. Addy, A. Bejsovec, and R. Saint (2005)
J. Cell Sci. 118, 5381-5392
   Abstract »    Full Text »    PDF »
A Rho-Type GTPase, rho-4, Is Required for Septation in Neurospora crassa.
C. G. Rasmussen and N. L. Glass (2005)
Eukaryot. Cell 4, 1913-1925
   Abstract »    Full Text »    PDF »
A WASp-binding type II phosphatidylinositol 4-kinase required for actin polymerization-driven endosome motility.
F. S. Chang, G.-S. Han, G. M. Carman, and K. J. Blumer (2005)
J. Cell Biol. 171, 133-142
   Abstract »    Full Text »    PDF »
Regulation of Cell Polarity by Interactions of Msb3 and Msb4 with Cdc42 and Polarisome Components.
S. E. Tcheperegine, X.-D. Gao, and E. Bi (2005)
Mol. Cell. Biol. 25, 8567-8580
   Abstract »    Full Text »    PDF »
Ras1-Induced Hyphal Development in Candida albicans Requires the Formin Bni1.
R. Martin, A. Walther, and J. Wendland (2005)
Eukaryot. Cell 4, 1712-1724
   Abstract »    Full Text »    PDF »
Distinct pathways control recruitment and maintenance of myosin II at the cleavage furrow during cytokinesis.
S. O. Dean, S. L. Rogers, N. Stuurman, R. D. Vale, and J. A. Spudich (2005)
PNAS 102, 13473-13478
   Abstract »    Full Text »    PDF »
The Formin Homology 1 Domain Modulates the Actin Nucleation and Bundling Activity of Arabidopsis FORMIN1.
A. Michelot, C. Guerin, S. Huang, M. Ingouff, S. Richard, N. Rodiuc, C. J. Staiger, and L. Blanchoin (2005)
PLANT CELL 17, 2296-2313
   Abstract »    Full Text »    PDF »
Differential Activities and Regulation of Saccharomyces cerevisiae Formin Proteins Bni1 and Bnr1 by Bud6.
J. B. Moseley and B. L. Goode (2005)
J. Biol. Chem. 280, 28023-28033
   Abstract »    Full Text »    PDF »
Candida albicans hyphae have a Spitzenkorper that is distinct from the polarisome found in yeast and pseudohyphae.
H. Crampin, K. Finley, M. Gerami-Nejad, H. Court, C. Gale, J. Berman, and P. Sudbery (2005)
J. Cell Sci. 118, 2935-2947
   Abstract »    Full Text »    PDF »
Fhos2, a novel formin-related actin-organizing protein, probably associates with the nestin intermediate filament.
H. Kanaya, R. Takeya, K. Takeuchi, N. Watanabe, N. Jing, and H. Sumimoto (2005)
Genes Cells 10, 665-678
   Abstract »    Full Text »    PDF »
RhoB regulates endosome transport by promoting actin assembly on endosomal membranes through Dia1.
M. Fernandez-Borja, L. Janssen, D. Verwoerd, P. Hordijk, and J. Neefjes (2005)
J. Cell Sci. 118, 2661-2670
   Abstract »    Full Text »    PDF »
The formin family protein CaBni1p has a role in cell polarity control during both yeast and hyphal growth in Candida albicans.
C. R. Li, Y. M. Wang, X. De Zheng, H. Y. Liang, J. C. W. Tang, and Y. Wang (2005)
J. Cell Sci. 118, 2637-2648
   Abstract »    Full Text »    PDF »
Cell Wall Integrity Signaling in Saccharomyces cerevisiae.
D. E. Levin (2005)
Microbiol. Mol. Biol. Rev. 69, 262-291
   Abstract »    Full Text »    PDF »
Cloning and Functional Characterization of a Formin-Like Protein (AtFH8) from Arabidopsis.
K. Yi, C. Guo, D. Chen, B. Zhao, B. Yang, and H. Ren (2005)
Plant Physiology 138, 1071-1082
   Abstract »    Full Text »    PDF »
Profilin-mediated Competition between Capping Protein and Formin Cdc12p during Cytokinesis in Fission Yeast.
D. R. Kovar, J.-Q. Wu, and T. D. Pollard (2005)
Mol. Biol. Cell 16, 2313-2324
   Abstract »    Full Text »    PDF »
The Molecular Requirements for Cytokinesis.
M. Glotzer (2005)
Science 307, 1735-1739
   Abstract »    Full Text »    PDF »
RhoGEF2 and the formin Dia control the formation of the furrow canal by directed actin assembly during Drosophila cellularisation.
J. Grosshans, C. Wenzl, H.-M. Herz, S. Bartoszewski, F. Schnorrer, N. Vogt, H. Schwarz, and H.-A. Muller (2005)
Development 132, 1009-1020
   Abstract »    Full Text »    PDF »
Dissecting Requirements for Auto-inhibition of Actin Nucleation by the Formin, mDia1.
F. Li and H. N. Higgs (2005)
J. Biol. Chem. 280, 6986-6992
   Abstract »    Full Text »    PDF »
Cofilin takes the lead.
V. DesMarais, M. Ghosh, R. Eddy, and J. Condeelis (2005)
J. Cell Sci. 118, 19-26
   Abstract »    Full Text »    PDF »
The role of the Rho GTPases in neuronal development.
E.-E. Govek, S. E. Newey, and L. Van Aelst (2005)
Genes & Dev. 19, 1-49
   Abstract »    Full Text »    PDF »
Nak1 interacts with Hob1 and Wsp1 to regulate cell growth and polarity in Schizosaccharomyces pombe.
T. Y. Huang, M. Renaud-Young, and D. Young (2005)
J. Cell Sci. 118, 199-210
   Abstract »    Full Text »    PDF »
Phylogenetic Analysis of the Formin Homology 2 Domain.
H. N. Higgs and K. J. Peterson (2005)
Mol. Biol. Cell 16, 1-13
   Abstract »    Full Text »    PDF »
Processive capping by formin suggests a force-driven mechanism of actin polymerization.
M. M. Kozlov and A. D. Bershadsky (2004)
J. Cell Biol. 167, 1011-1017
   Abstract »    Full Text »    PDF »
Septin Ring Assembly Requires Concerted Action of Polarisome Components, a PAK Kinase Cla4p, and the Actin Cytoskeleton in Saccharomyces cerevisiae.
J. Kadota, T. Yamamoto, S. Yoshiuchi, E. Bi, and K. Tanaka (2004)
Mol. Biol. Cell 15, 5329-5345
   Abstract »    Full Text »    PDF »
Homo-oligomerization Is Essential for F-actin Assembly by the Formin Family FH2 Domain.
J. W. Copeland, S. J. Copeland, and R. Treisman (2004)
J. Biol. Chem. 279, 50250-50256
   Abstract »    Full Text »    PDF »
Stable and Dynamic Axes of Polarity Use Distinct Formin Isoforms in Budding Yeast.
D. Pruyne, L. Gao, E. Bi, and A. Bretscher (2004)
Mol. Biol. Cell 15, 4971-4989
   Abstract »    Full Text »    PDF »
From the Cover: Insertional assembly of actin filament barbed ends in association with formins produces piconewton forces.
D. R. Kovar and T. D. Pollard (2004)
PNAS 101, 14725-14730
   Abstract »    Full Text »    PDF »
Apical localization of actin patches and vacuolar dynamics in Ashbya gossypii depend on the WASP homolog Wal1p.
A. Walther and J. Wendland (2004)
J. Cell Sci. 117, 4947-4958
   Abstract »    Full Text »    PDF »
Arabidopsis Formin AtFH6 Is a Plasma Membrane-Associated Protein Upregulated in Giant Cells Induced by Parasitic Nematodes.
B. Favery, L. A. Chelysheva, M. Lebris, F. Jammes, A. Marmagne, J. de Almeida-Engler, P. Lecomte, C. Vaury, R. A. Arkowitz, and P. Abad (2004)
PLANT CELL 16, 2529-2540
   Abstract »    Full Text »    PDF »
MesA, a Novel Fungal Protein Required for the Stabilization of Polarity Axes in Aspergillus nidulans.
C. L. Pearson, K. Xu, K. E. Sharpless, and S. D. Harris (2004)
Mol. Biol. Cell 15, 3658-3672
   Abstract »    Full Text »    PDF »
Regulation of a formin complex by the microtubule plus end protein tea1p.
B. Feierbach, F. Verde, and F. Chang (2004)
J. Cell Biol. 165, 697-707
   Abstract »    Full Text »    PDF »
EBV attachment stimulates FHOS/FHOD1 redistribution and co-aggregation with CD21: formin interactions with the cytoplasmic domain of human CD21.
M. B. Gill, J. Roecklein-Canfield, D. R. Sage, M. Zambela-Soediono, N. Longtine, M. Uknis, and J. D. Fingeroth (2004)
J. Cell Sci. 117, 2709-2720
   Abstract »    Full Text »    PDF »
The Mouse Formin, FRL{alpha}, Slows Actin Filament Barbed End Elongation, Competes with Capping Protein, Accelerates Polymerization from Monomers, and Severs Filaments.
E. S. Harris, F. Li, and H. N. Higgs (2004)
J. Biol. Chem. 279, 20076-20087
   Abstract »    Full Text »    PDF »
Pheromone-induced polarization is dependent on the Fus3p MAPK acting through the formin Bni1p.
D. Matheos, M. Metodiev, E. Muller, D. Stone, and M. D. Rose (2004)
J. Cell Biol. 165, 99-109
   Abstract »    Full Text »    PDF »
Polarized Hyphal Growth in Candida albicans Requires the Wiskott-Aldrich Syndrome Protein Homolog Wal1p.
A. Walther and J. Wendland (2004)
Eukaryot. Cell 3, 471-482
   Abstract »    Full Text »    PDF »
Actin Polymerization-Driven Molecular Movement of mDia1 in Living Cells.
C. Higashida, T. Miyoshi, A. Fujita, F. Oceguera-Yanez, J. Monypenny, Y. Andou, S. Narumiya, and N. Watanabe (2004)
Science 303, 2007-2010
   Abstract »    Full Text »    PDF »
Lessons from the Genome Sequence of Neurospora crassa: Tracing the Path from Genomic Blueprint to Multicellular Organism.
K. A. Borkovich, L. A. Alex, O. Yarden, M. Freitag, G. E. Turner, N. D. Read, S. Seiler, D. Bell-Pedersen, J. Paietta, N. Plesofsky, et al. (2004)
Microbiol. Mol. Biol. Rev. 68, 1-108
   Abstract »    Full Text »    PDF »
A Conserved Mechanism for Bni1- and mDia1-induced Actin Assembly and Dual Regulation of Bni1 by Bud6 and Profilin.
J. B. Moseley, I. Sagot, A. L. Manning, Y. Xu, M. J. Eck, D. Pellman, and B. L. Goode (2004)
Mol. Biol. Cell 15, 896-907
   Abstract »    Full Text »    PDF »
Overexpression of an Arabidopsis Formin Stimulates Supernumerary Actin Cable Formation from Pollen Tube Cell Membrane.
A. Y. Cheung and H.-m. Wu (2004)
PLANT CELL 16, 257-269
   Abstract »    Full Text »    PDF »
Fhos, a mammalian formin, directly binds to F-actin via a region N-terminal to the FH1 domain and forms a homotypic complex via the FH2 domain to promote actin fiber formation.
R. Takeya and H. Sumimoto (2003)
J. Cell Sci. 116, 4567-4575
   Abstract »    Full Text »    PDF »
A Role for GEA1 and GEA2 in the Organization of the Actin Cytoskeleton in Saccharomyces cerevisiae.
E. Zakrzewska, M. Perron, A. Laroche, and D. Pallotta (2003)
Genetics 165, 985-995
   Abstract »    Full Text »    PDF »
Activation of the Rac-binding Partner FHOD1 Induces Actin Stress Fibers via a ROCK-dependent Mechanism.
J. E. Gasteier, R. Madrid, E. Krautkramer, S. Schroder, W. Muranyi, S. Benichou, and O. T. Fackler (2003)
J. Biol. Chem. 278, 38902-38912
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)