Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Global Protein Stability Profiling in Mammalian Cells
Hsueh-Chi Sherry Yen,Qikai Xu,*Danny M. Chou,*Zhenming Zhao,Stephen J. Elledge
The abundance of cellular proteins is determined largely bythe rate of transcription and translation coupled with the stabilityof individual proteins. Although we know a great deal aboutglobal transcript abundance, little is known about global proteinstability. We present a highly parallel multiplexing strategyto monitor protein turnover on a global scale by coupling flowcytometry with microarray technology to track the stabilityof individual proteins within a complex mixture. We demonstratedthe feasibility of this approach by measuring the stabilityof 8000 human proteins and identifying proteasome substrates.The technology provides a general platform for proteome-scaleanalysis of protein turnover under various physiological anddisease conditions.
Department of Genetics, Center for Genetics and Genomics, Brigham and Women's Hospital, Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA.
* These authors contributed equally to this work.
To whom correspondence should be addressed. E-mail: selledge{at}genetics.med.harvard.edu
L. Bryan Ray (11 November 2008) Sci. Signal.1 (45), ec388.
[DOI: 10.1126/scisignal.145ec388] |Abstract »
THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Aberrant DNA Polymerase {alpha} Is Excluded from the Nucleus by Defective Import and Degradation in the Nucleus.
C. S. Eichinger, T. Mizuno, K. Mizuno, Y. Miyake, K.-i. Yanagi, N. Imamoto, and F. Hanaoka (2009)
J. Biol. Chem.
284, 30604-30614
|Abstract »|Full Text »|PDF »
Cotranslational Proteolysis Dominates Glutathione Homeostasis to Support Proper Growth and Development.
F. Frottin, C. Espagne, J. A. Traverso, C. Mauve, B. Valot, C. Lelarge-Trouverie, M. Zivy, G. Noctor, T. Meinnel, and C. Giglione (2009)
PLANT CELL
21, 3296-3314
|Abstract »|Full Text »|PDF »
Hypoxia-inducible Factor Prolyl-4-hydroxylase PHD2 Protein Abundance Depends on Integral Membrane Anchoring of FKBP38.
S. Barth, F. Edlich, U. Berchner-Pfannschmidt, S. Gneuss, G. Jahreis, P. A. Hasgall, J. Fandrey, R. H. Wenger, and G. Camenisch (2009)
J. Biol. Chem.
284, 23046-23058
|Abstract »|Full Text »|PDF »
Analysis of Post-transcriptional Regulations by a Functional, Integrated, and Quantitative Method.
B. Laloo, D. Simon, V. Veillat, D. Lauzel, V. Guyonnet-Duperat, F. Moreau-Gaudry, F. Sagliocco, and C. Grosset (2009)
Mol. Cell. Proteomics
8, 1777-1788
|Abstract »|Full Text »|PDF »
ROC1/RBX1 E3 Ubiquitin Ligase Silencing Suppresses Tumor Cell Growth via Sequential Induction of G2-M Arrest, Apoptosis, and Senescence.
8000 human proteins and identifying proteasome substrates. The technology provides a general platform for proteome-scale analysis of protein turnover under various physiological and disease conditions. 
To whom correspondence should be addressed. E-mail: 
